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Image Search Results
Journal: Oncology Reports
Article Title: MicroRNA-188-5p inhibits the progression of breast cancer by targeting zinc finger protein 91
doi: 10.3892/or.2020.7731
Figure Lengend Snippet: miR-188-5p is downregulated in BC tissue and BC cell lines. (A) RT-qPCR results showed that the level of miR-188-5p in BC tissues was downregulated compared with counterpart BC tissues. The comparisons between groups were evaluated by Student's t-test. (B) Relative expression level of miR-188-5p in patients at different clinical stages. (C) Relative expression level of miR-188-5p in BC cell lines MDA-MB-231, BT-549 and MCF-7 relative to the normal human breast epithelial cell line MCF-10A. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B and C. All data are presented as means ± SD (n=3). BC, breast cancer.
Article Snippet: The primers for
Techniques: Quantitative RT-PCR, Expressing
Journal: Oncology Reports
Article Title: MicroRNA-188-5p inhibits the progression of breast cancer by targeting zinc finger protein 91
doi: 10.3892/or.2020.7731
Figure Lengend Snippet: Effect of miR-188-5p on BC cell line MDA-MB-231. MDA-MB-231 cells were transfected with miR-188-5p mimics and miR-NC. (A) The mRNA levels of miR-188-5p in MDA-MB-231 cells were determined by RT-qPCR. *P<0.05. The proliferation of MDA-MB-231 cells was examined by (B) CCK-8 Kit post transfection and (C) colony formation. *P<0.05, compared to control group; # P<0.05, compared to miR-NC group. (D) The apoptotic MDA-MB-231 cells were analyzed using Annexin V/PI staining and FACs. *P<0.05. (E) The invasion and migration capability of MDA-MB-231 cells were detected by Transwell assay. *P<0.05. (F) Expression of E-cadherin, N-cadherin, vimentin, MMP2, MMP9 and NF-κB p65(Rel) were detected by western blotting. The data are represented as the mean ± SD (n=3). *P<0.05. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons. BC, breast cancer; MMP, matrix metalloproteinase; NC, negative control.
Article Snippet: The primers for
Techniques: Transfection, Quantitative RT-PCR, CCK-8 Assay, Staining, Migration, Transwell Assay, Expressing, Western Blot, Negative Control
Journal: Oncology Reports
Article Title: MicroRNA-188-5p inhibits the progression of breast cancer by targeting zinc finger protein 91
doi: 10.3892/or.2020.7731
Figure Lengend Snippet: ZFP91 is a target of miR-188-5p. (A) ZFP91 provides binding sites with miR-188-5p. (B) RT-qPCR and western blotting were used for analysis of transcription and translation of ZFP91 in vitro and in vivo . *P<0.05. (C) The binding between miR-188-5p and ZFP91 was confirmed by luciferase assay. *P<0.05. Relative gene expression was normalized by GAPDH expression. The data are represented as the mean ± SD (n=3). One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B. The comparisons between two groups were evaluated by t-test in C. ZFP91, zinc finger protein 91.
Article Snippet: The primers for
Techniques: Binding Assay, Quantitative RT-PCR, Western Blot, In Vitro, In Vivo, Luciferase, Expressing
Journal: Oncology Reports
Article Title: MicroRNA-188-5p inhibits the progression of breast cancer by targeting zinc finger protein 91
doi: 10.3892/or.2020.7731
Figure Lengend Snippet: The regulatory role of miR-188-5p in a BC xenograft mouse model. BC MDA-MB-231 cells were transfected with miR-188-5p mimics and miR-NC, and then injected into nude mice. (A) The tumor volume and weight in the tumor xenograft mouse were compared among the miR-188-5p mimics group, miR-NC group and control group. *P<0.05, compared to control group; # P<0.05, compared to miR-NC group. (B) The protein expression and mRNA level of ZPF91 were detected by (C) western blotting and RT-qPCR. The data are represented as the mean ± SD (n=3). *P<0.05. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons. BC, breast cancer; ZFP91, zinc finger protein 91; NC, negative control.
Article Snippet: The primers for
Techniques: Transfection, Injection, Expressing, Western Blot, Quantitative RT-PCR, Negative Control
Journal: Oncology Reports
Article Title: MicroRNA-188-5p inhibits the progression of breast cancer by targeting zinc finger protein 91
doi: 10.3892/or.2020.7731
Figure Lengend Snippet: MDA-MB-231 cell proliferation and apoptosis are regulated by miR-188-5p/ZFP91. pCMV-Tag2B vector (PC) was transfected into BC MDA-MB-231 cells with miR-NC (NC+PC) or miR-188-5p mimics (PC+miR-188-5p); pCMV-Tag2B-ZFP91 was transfected into MDA-MB-231 cells with miR-188-5p mimics (miR-188-5p+ZFP91). (A) The mRNA levels of miR-188-5p in the co-transfected overexpressing ZFP91/MDA-MB-231 cells were quantified by RT-qPCR. *P<0.05. Cell proliferation was measured by (B) the CCK-8 Kit and (C) colony formation assay. *P<0.05, compared to control group; # P<0.05, compared to miR-NC group. (D) Cell apoptosis was detected by Annexin V/PI staining and FACs. *P<0.05. (E) The invasion and migration capability of MDA-MB-231 cells were detected by Transwell assay. *P<0.05. (F) Expression of E-cadherin, N-cadherin, Vimentin, MMP2, MMP9 and NF-κB p65(Rel) were detected by western blotting. Relative genes expression was normalized by GAPDH expression. The data are represented as the mean ± SD (n=3). *P<0.05. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons. BC, breast cancer; ZFP91, zinc finger protein 91; MMP, matrix metalloproteinase; NC, negative control.
Article Snippet: The primers for
Techniques: Plasmid Preparation, Transfection, Quantitative RT-PCR, CCK-8 Assay, Colony Assay, Staining, Migration, Transwell Assay, Expressing, Western Blot, Negative Control
Journal: Oncology Reports
Article Title: MicroRNA-188-5p inhibits the progression of breast cancer by targeting zinc finger protein 91
doi: 10.3892/or.2020.7731
Figure Lengend Snippet: mRNA and protein levels of ZPF91 in BC tissues. (A) The mRNA and protein levels of ZPF91 in BC tissue and corresponding non-tumor normal tissue were quantified using RT-qPCR and western blot analysis. The comparisons between two groups were evaluated by t-test. N, non-cancer tissue; C, cancer tissue. *P<0.05. (B) Correlation between ZPF91 mRNA and miR-188-5p was analyzed using Pearson's correlation coefficient. The data are represented as the mean ± SD (n=100). BC, breast cancer; ZFP91, zinc finger protein 91.
Article Snippet: The primers for
Techniques: Quantitative RT-PCR, Western Blot