mir gene Search Results


90
Nihon Gene Research Laboratories mir-499 mimic
Mir 499 Mimic, supplied by Nihon Gene Research Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biopharm GmbH the primers for mir-188-5p and the reference gene u6
<t>miR-188-5p</t> is downregulated in BC tissue and BC cell lines. (A) RT-qPCR results showed that the level of miR-188-5p in BC tissues was downregulated compared with counterpart BC tissues. The comparisons between groups were evaluated by Student's t-test. (B) Relative expression level of miR-188-5p in patients at different clinical stages. (C) Relative expression level of miR-188-5p in BC cell lines MDA-MB-231, BT-549 and MCF-7 relative to the normal human breast epithelial cell line MCF-10A. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B and C. All data are presented as means ± SD (n=3). BC, breast cancer.
The Primers For Mir 188 5p And The Reference Gene U6, supplied by Biopharm GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
GenScript corporation mir-998 gene
<t>miR-188-5p</t> is downregulated in BC tissue and BC cell lines. (A) RT-qPCR results showed that the level of miR-188-5p in BC tissues was downregulated compared with counterpart BC tissues. The comparisons between groups were evaluated by Student's t-test. (B) Relative expression level of miR-188-5p in patients at different clinical stages. (C) Relative expression level of miR-188-5p in BC cell lines MDA-MB-231, BT-549 and MCF-7 relative to the normal human breast epithelial cell line MCF-10A. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B and C. All data are presented as means ± SD (n=3). BC, breast cancer.
Mir 998 Gene, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Ribobio co gene and mir taqman qrt-pcr specific probes
<t>miR-188-5p</t> is downregulated in BC tissue and BC cell lines. (A) RT-qPCR results showed that the level of miR-188-5p in BC tissues was downregulated compared with counterpart BC tissues. The comparisons between groups were evaluated by Student's t-test. (B) Relative expression level of miR-188-5p in patients at different clinical stages. (C) Relative expression level of miR-188-5p in BC cell lines MDA-MB-231, BT-549 and MCF-7 relative to the normal human breast epithelial cell line MCF-10A. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B and C. All data are presented as means ± SD (n=3). BC, breast cancer.
Gene And Mir Taqman Qrt Pcr Specific Probes, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Phalanx Biotech mir and mrna-gene expression analyses
<t>miR-188-5p</t> is downregulated in BC tissue and BC cell lines. (A) RT-qPCR results showed that the level of miR-188-5p in BC tissues was downregulated compared with counterpart BC tissues. The comparisons between groups were evaluated by Student's t-test. (B) Relative expression level of miR-188-5p in patients at different clinical stages. (C) Relative expression level of miR-188-5p in BC cell lines MDA-MB-231, BT-549 and MCF-7 relative to the normal human breast epithelial cell line MCF-10A. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B and C. All data are presented as means ± SD (n=3). BC, breast cancer.
Mir And Mrna Gene Expression Analyses, supplied by Phalanx Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nihon Gene Research Laboratories mir-181a1 mimic
<t>miR-188-5p</t> is downregulated in BC tissue and BC cell lines. (A) RT-qPCR results showed that the level of miR-188-5p in BC tissues was downregulated compared with counterpart BC tissues. The comparisons between groups were evaluated by Student's t-test. (B) Relative expression level of miR-188-5p in patients at different clinical stages. (C) Relative expression level of miR-188-5p in BC cell lines MDA-MB-231, BT-549 and MCF-7 relative to the normal human breast epithelial cell line MCF-10A. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B and C. All data are presented as means ± SD (n=3). BC, breast cancer.
Mir 181a1 Mimic, supplied by Nihon Gene Research Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mir+gene/mir+181a1+mimic/pm37968862-133-19-42
Average 90 stars, based on 1 article reviews
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90
Ribobio co mir rb reporttm dual luciferase reporter gene plasmid vector
<t>miR-188-5p</t> is downregulated in BC tissue and BC cell lines. (A) RT-qPCR results showed that the level of miR-188-5p in BC tissues was downregulated compared with counterpart BC tissues. The comparisons between groups were evaluated by Student's t-test. (B) Relative expression level of miR-188-5p in patients at different clinical stages. (C) Relative expression level of miR-188-5p in BC cell lines MDA-MB-231, BT-549 and MCF-7 relative to the normal human breast epithelial cell line MCF-10A. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B and C. All data are presented as means ± SD (n=3). BC, breast cancer.
Mir Rb Reporttm Dual Luciferase Reporter Gene Plasmid Vector, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mir+gene/mir+rb+reporttm+dual+luciferase+reporter+gene+plasmid+vector/pmc06834497-98-25-35
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90
Broad Institute Inc illuminahiseq_mirnaseq-mir_gene_expression
<t>miR-188-5p</t> is downregulated in BC tissue and BC cell lines. (A) RT-qPCR results showed that the level of miR-188-5p in BC tissues was downregulated compared with counterpart BC tissues. The comparisons between groups were evaluated by Student's t-test. (B) Relative expression level of miR-188-5p in patients at different clinical stages. (C) Relative expression level of miR-188-5p in BC cell lines MDA-MB-231, BT-549 and MCF-7 relative to the normal human breast epithelial cell line MCF-10A. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B and C. All data are presented as means ± SD (n=3). BC, breast cancer.
Illuminahiseq Mirnaseq Mir Gene Expression, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mir+gene/illuminahiseq+mirnaseq+mir+gene+expression/pmc06359980-71-5-22
Average 90 stars, based on 1 article reviews
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94
Thermo Fisher mir 196a2 rs11614913 c t genes polymorphisms
<t>miR-188-5p</t> is downregulated in BC tissue and BC cell lines. (A) RT-qPCR results showed that the level of miR-188-5p in BC tissues was downregulated compared with counterpart BC tissues. The comparisons between groups were evaluated by Student's t-test. (B) Relative expression level of miR-188-5p in patients at different clinical stages. (C) Relative expression level of miR-188-5p in BC cell lines MDA-MB-231, BT-549 and MCF-7 relative to the normal human breast epithelial cell line MCF-10A. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B and C. All data are presented as means ± SD (n=3). BC, breast cancer.
Mir 196a2 Rs11614913 C T Genes Polymorphisms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Informa UK Limited mir-146a-knocked out mice
<t>miR-188-5p</t> is downregulated in BC tissue and BC cell lines. (A) RT-qPCR results showed that the level of miR-188-5p in BC tissues was downregulated compared with counterpart BC tissues. The comparisons between groups were evaluated by Student's t-test. (B) Relative expression level of miR-188-5p in patients at different clinical stages. (C) Relative expression level of miR-188-5p in BC cell lines MDA-MB-231, BT-549 and MCF-7 relative to the normal human breast epithelial cell line MCF-10A. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B and C. All data are presented as means ± SD (n=3). BC, breast cancer.
Mir 146a Knocked Out Mice, supplied by Informa UK Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mir+gene/mir+146a+gene/pm33155514-20-10-22
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90
Epigenomics ag mir-210 gene expression
<t>miR-188-5p</t> is downregulated in BC tissue and BC cell lines. (A) RT-qPCR results showed that the level of miR-188-5p in BC tissues was downregulated compared with counterpart BC tissues. The comparisons between groups were evaluated by Student's t-test. (B) Relative expression level of miR-188-5p in patients at different clinical stages. (C) Relative expression level of miR-188-5p in BC cell lines MDA-MB-231, BT-549 and MCF-7 relative to the normal human breast epithelial cell line MCF-10A. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B and C. All data are presented as means ± SD (n=3). BC, breast cancer.
Mir 210 Gene Expression, supplied by Epigenomics ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mir+gene/mir+210+gene+expression/pm30109809-105-13-1
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86
Strem Chemicals gene mir 5571 3p
<t>miR-188-5p</t> is downregulated in BC tissue and BC cell lines. (A) RT-qPCR results showed that the level of miR-188-5p in BC tissues was downregulated compared with counterpart BC tissues. The comparisons between groups were evaluated by Student's t-test. (B) Relative expression level of miR-188-5p in patients at different clinical stages. (C) Relative expression level of miR-188-5p in BC cell lines MDA-MB-231, BT-549 and MCF-7 relative to the normal human breast epithelial cell line MCF-10A. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B and C. All data are presented as means ± SD (n=3). BC, breast cancer.
Gene Mir 5571 3p, supplied by Strem Chemicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


miR-188-5p is downregulated in BC tissue and BC cell lines. (A) RT-qPCR results showed that the level of miR-188-5p in BC tissues was downregulated compared with counterpart BC tissues. The comparisons between groups were evaluated by Student's t-test. (B) Relative expression level of miR-188-5p in patients at different clinical stages. (C) Relative expression level of miR-188-5p in BC cell lines MDA-MB-231, BT-549 and MCF-7 relative to the normal human breast epithelial cell line MCF-10A. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B and C. All data are presented as means ± SD (n=3). BC, breast cancer.

Journal: Oncology Reports

Article Title: MicroRNA-188-5p inhibits the progression of breast cancer by targeting zinc finger protein 91

doi: 10.3892/or.2020.7731

Figure Lengend Snippet: miR-188-5p is downregulated in BC tissue and BC cell lines. (A) RT-qPCR results showed that the level of miR-188-5p in BC tissues was downregulated compared with counterpart BC tissues. The comparisons between groups were evaluated by Student's t-test. (B) Relative expression level of miR-188-5p in patients at different clinical stages. (C) Relative expression level of miR-188-5p in BC cell lines MDA-MB-231, BT-549 and MCF-7 relative to the normal human breast epithelial cell line MCF-10A. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B and C. All data are presented as means ± SD (n=3). BC, breast cancer.

Article Snippet: The primers for miR-188-5p and the reference gene U6 were purchased from Novland Biopharm.

Techniques: Quantitative RT-PCR, Expressing

Effect of miR-188-5p on BC cell line MDA-MB-231. MDA-MB-231 cells were transfected with miR-188-5p mimics and miR-NC. (A) The mRNA levels of miR-188-5p in MDA-MB-231 cells were determined by RT-qPCR. *P<0.05. The proliferation of MDA-MB-231 cells was examined by (B) CCK-8 Kit post transfection and (C) colony formation. *P<0.05, compared to control group; # P<0.05, compared to miR-NC group. (D) The apoptotic MDA-MB-231 cells were analyzed using Annexin V/PI staining and FACs. *P<0.05. (E) The invasion and migration capability of MDA-MB-231 cells were detected by Transwell assay. *P<0.05. (F) Expression of E-cadherin, N-cadherin, vimentin, MMP2, MMP9 and NF-κB p65(Rel) were detected by western blotting. The data are represented as the mean ± SD (n=3). *P<0.05. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons. BC, breast cancer; MMP, matrix metalloproteinase; NC, negative control.

Journal: Oncology Reports

Article Title: MicroRNA-188-5p inhibits the progression of breast cancer by targeting zinc finger protein 91

doi: 10.3892/or.2020.7731

Figure Lengend Snippet: Effect of miR-188-5p on BC cell line MDA-MB-231. MDA-MB-231 cells were transfected with miR-188-5p mimics and miR-NC. (A) The mRNA levels of miR-188-5p in MDA-MB-231 cells were determined by RT-qPCR. *P<0.05. The proliferation of MDA-MB-231 cells was examined by (B) CCK-8 Kit post transfection and (C) colony formation. *P<0.05, compared to control group; # P<0.05, compared to miR-NC group. (D) The apoptotic MDA-MB-231 cells were analyzed using Annexin V/PI staining and FACs. *P<0.05. (E) The invasion and migration capability of MDA-MB-231 cells were detected by Transwell assay. *P<0.05. (F) Expression of E-cadherin, N-cadherin, vimentin, MMP2, MMP9 and NF-κB p65(Rel) were detected by western blotting. The data are represented as the mean ± SD (n=3). *P<0.05. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons. BC, breast cancer; MMP, matrix metalloproteinase; NC, negative control.

Article Snippet: The primers for miR-188-5p and the reference gene U6 were purchased from Novland Biopharm.

Techniques: Transfection, Quantitative RT-PCR, CCK-8 Assay, Staining, Migration, Transwell Assay, Expressing, Western Blot, Negative Control

ZFP91 is a target of miR-188-5p. (A) ZFP91 provides binding sites with miR-188-5p. (B) RT-qPCR and western blotting were used for analysis of transcription and translation of ZFP91 in vitro and in vivo . *P<0.05. (C) The binding between miR-188-5p and ZFP91 was confirmed by luciferase assay. *P<0.05. Relative gene expression was normalized by GAPDH expression. The data are represented as the mean ± SD (n=3). One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B. The comparisons between two groups were evaluated by t-test in C. ZFP91, zinc finger protein 91.

Journal: Oncology Reports

Article Title: MicroRNA-188-5p inhibits the progression of breast cancer by targeting zinc finger protein 91

doi: 10.3892/or.2020.7731

Figure Lengend Snippet: ZFP91 is a target of miR-188-5p. (A) ZFP91 provides binding sites with miR-188-5p. (B) RT-qPCR and western blotting were used for analysis of transcription and translation of ZFP91 in vitro and in vivo . *P<0.05. (C) The binding between miR-188-5p and ZFP91 was confirmed by luciferase assay. *P<0.05. Relative gene expression was normalized by GAPDH expression. The data are represented as the mean ± SD (n=3). One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons in B. The comparisons between two groups were evaluated by t-test in C. ZFP91, zinc finger protein 91.

Article Snippet: The primers for miR-188-5p and the reference gene U6 were purchased from Novland Biopharm.

Techniques: Binding Assay, Quantitative RT-PCR, Western Blot, In Vitro, In Vivo, Luciferase, Expressing

The regulatory role of miR-188-5p in a BC xenograft mouse model. BC MDA-MB-231 cells were transfected with miR-188-5p mimics and miR-NC, and then injected into nude mice. (A) The tumor volume and weight in the tumor xenograft mouse were compared among the miR-188-5p mimics group, miR-NC group and control group. *P<0.05, compared to control group; # P<0.05, compared to miR-NC group. (B) The protein expression and mRNA level of ZPF91 were detected by (C) western blotting and RT-qPCR. The data are represented as the mean ± SD (n=3). *P<0.05. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons. BC, breast cancer; ZFP91, zinc finger protein 91; NC, negative control.

Journal: Oncology Reports

Article Title: MicroRNA-188-5p inhibits the progression of breast cancer by targeting zinc finger protein 91

doi: 10.3892/or.2020.7731

Figure Lengend Snippet: The regulatory role of miR-188-5p in a BC xenograft mouse model. BC MDA-MB-231 cells were transfected with miR-188-5p mimics and miR-NC, and then injected into nude mice. (A) The tumor volume and weight in the tumor xenograft mouse were compared among the miR-188-5p mimics group, miR-NC group and control group. *P<0.05, compared to control group; # P<0.05, compared to miR-NC group. (B) The protein expression and mRNA level of ZPF91 were detected by (C) western blotting and RT-qPCR. The data are represented as the mean ± SD (n=3). *P<0.05. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons. BC, breast cancer; ZFP91, zinc finger protein 91; NC, negative control.

Article Snippet: The primers for miR-188-5p and the reference gene U6 were purchased from Novland Biopharm.

Techniques: Transfection, Injection, Expressing, Western Blot, Quantitative RT-PCR, Negative Control

MDA-MB-231 cell proliferation and apoptosis are regulated by miR-188-5p/ZFP91. pCMV-Tag2B vector (PC) was transfected into BC MDA-MB-231 cells with miR-NC (NC+PC) or miR-188-5p mimics (PC+miR-188-5p); pCMV-Tag2B-ZFP91 was transfected into MDA-MB-231 cells with miR-188-5p mimics (miR-188-5p+ZFP91). (A) The mRNA levels of miR-188-5p in the co-transfected overexpressing ZFP91/MDA-MB-231 cells were quantified by RT-qPCR. *P<0.05. Cell proliferation was measured by (B) the CCK-8 Kit and (C) colony formation assay. *P<0.05, compared to control group; # P<0.05, compared to miR-NC group. (D) Cell apoptosis was detected by Annexin V/PI staining and FACs. *P<0.05. (E) The invasion and migration capability of MDA-MB-231 cells were detected by Transwell assay. *P<0.05. (F) Expression of E-cadherin, N-cadherin, Vimentin, MMP2, MMP9 and NF-κB p65(Rel) were detected by western blotting. Relative genes expression was normalized by GAPDH expression. The data are represented as the mean ± SD (n=3). *P<0.05. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons. BC, breast cancer; ZFP91, zinc finger protein 91; MMP, matrix metalloproteinase; NC, negative control.

Journal: Oncology Reports

Article Title: MicroRNA-188-5p inhibits the progression of breast cancer by targeting zinc finger protein 91

doi: 10.3892/or.2020.7731

Figure Lengend Snippet: MDA-MB-231 cell proliferation and apoptosis are regulated by miR-188-5p/ZFP91. pCMV-Tag2B vector (PC) was transfected into BC MDA-MB-231 cells with miR-NC (NC+PC) or miR-188-5p mimics (PC+miR-188-5p); pCMV-Tag2B-ZFP91 was transfected into MDA-MB-231 cells with miR-188-5p mimics (miR-188-5p+ZFP91). (A) The mRNA levels of miR-188-5p in the co-transfected overexpressing ZFP91/MDA-MB-231 cells were quantified by RT-qPCR. *P<0.05. Cell proliferation was measured by (B) the CCK-8 Kit and (C) colony formation assay. *P<0.05, compared to control group; # P<0.05, compared to miR-NC group. (D) Cell apoptosis was detected by Annexin V/PI staining and FACs. *P<0.05. (E) The invasion and migration capability of MDA-MB-231 cells were detected by Transwell assay. *P<0.05. (F) Expression of E-cadherin, N-cadherin, Vimentin, MMP2, MMP9 and NF-κB p65(Rel) were detected by western blotting. Relative genes expression was normalized by GAPDH expression. The data are represented as the mean ± SD (n=3). *P<0.05. One-way ANOVA followed by Tukey's test was used to evaluate the difference for multiple comparisons. BC, breast cancer; ZFP91, zinc finger protein 91; MMP, matrix metalloproteinase; NC, negative control.

Article Snippet: The primers for miR-188-5p and the reference gene U6 were purchased from Novland Biopharm.

Techniques: Plasmid Preparation, Transfection, Quantitative RT-PCR, CCK-8 Assay, Colony Assay, Staining, Migration, Transwell Assay, Expressing, Western Blot, Negative Control

mRNA and protein levels of ZPF91 in BC tissues. (A) The mRNA and protein levels of ZPF91 in BC tissue and corresponding non-tumor normal tissue were quantified using RT-qPCR and western blot analysis. The comparisons between two groups were evaluated by t-test. N, non-cancer tissue; C, cancer tissue. *P<0.05. (B) Correlation between ZPF91 mRNA and miR-188-5p was analyzed using Pearson's correlation coefficient. The data are represented as the mean ± SD (n=100). BC, breast cancer; ZFP91, zinc finger protein 91.

Journal: Oncology Reports

Article Title: MicroRNA-188-5p inhibits the progression of breast cancer by targeting zinc finger protein 91

doi: 10.3892/or.2020.7731

Figure Lengend Snippet: mRNA and protein levels of ZPF91 in BC tissues. (A) The mRNA and protein levels of ZPF91 in BC tissue and corresponding non-tumor normal tissue were quantified using RT-qPCR and western blot analysis. The comparisons between two groups were evaluated by t-test. N, non-cancer tissue; C, cancer tissue. *P<0.05. (B) Correlation between ZPF91 mRNA and miR-188-5p was analyzed using Pearson's correlation coefficient. The data are represented as the mean ± SD (n=100). BC, breast cancer; ZFP91, zinc finger protein 91.

Article Snippet: The primers for miR-188-5p and the reference gene U6 were purchased from Novland Biopharm.

Techniques: Quantitative RT-PCR, Western Blot